Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/NRR.NRR-D-23-01881

Figure Lengend Snippet: Overview of the pharmacological treatments performed in adult zebrafish

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Concentration Assay, Liposomes, Saline

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/NRR.NRR-D-23-01881

Figure Lengend Snippet: Analysis of regenerative angiogenesis and neurogenesis after VEGF165 injection. (A) PCNA immunohistostaining (proliferating cells in purple) in telencephalon sections of Tg( fli:EGFP × mpeg1.1:mCherry ) zebrafish (blood vessels in green) after microinjection with PBS (vehicle) and VEGF165 (50 µg/mL) at the SW injury site. Scale bars: 200 µm. (B–D) Quantification of different angiogenic parameters shows an increasing regenerative angiogenesis tendency in the SW hemisphere of VEGF165-injected zebrafish compared with the SW hemisphere of PBS-injected zebrafish at 1 dpl. (E) Quantification of the ventricular proliferative surface of fish injected with PBS and VEGF165 showing an increasing tendency for NSC proliferation. The data are presented as the mean ± standard error of the mean ( n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t -test. * P < 0.05, vs . the SW hemisphere of PBS-injected zebrafish. dpl: Day(s) post-lesion; EGFP: enhanced green fluorescent protein; GFP: green fluorescent protein; PBS: phosphate-buffered saline; PCNA: proliferating cell nuclear antigen; SW: stab-wounded; VEGF: vascular endothelial growth factor.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Injection, Microinjection, Saline

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/NRR.NRR-D-23-01881

Figure Lengend Snippet: Microglial recruitment is regulated by Vegf signaling. The SW hemisphere was compared with the respective contralateral hemisphere at 3 dpl. (A, C) Telencephalon sections of Tg( fli:EGFP × mpeg1.1:mCherry ) zebrafish (microglia in red). Scale bars: 200 µm. (B) Quantification of mpeg + cells showing a decrease in tivozanib-injected zebrafish compared with DMSO-injected zebrafish. (D) Quantification of mpeg + cells showing an increasing tendency in VEGF165-injected zebrafish compared with PBS-injected zebrafish. The data are presented as the mean ± standard error of the mean ( n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t -test. *** P < 0.001, vs . the contralateral hemisphere. DMSO: Dimethyl sulfoxide; dpl: day(s) post-lesion; mpeg: macrophage‐expressed gene; PBS: phosphate-buffered saline; SW: stab-wounded; Vegf/VEGF: vascular endothelial growth factor.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Injection, Saline

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/NRR.NRR-D-23-01881

Figure Lengend Snippet: Microglial and endothelial cell proliferation following brain lesion and modulation of Vegf signaling. (A) Representative images of PCNA immunostaining of Tg( fli:EGFP × mpeg1.1:mCherry ) zebrafish following microlesion/injection with DAPI counterstaining. (B) Higher magnification of the white panels in A illustrating endothelial cell proliferation (Fli + /PCNA + ) and microglial proliferation (mpeg + /PCNA + ). Scale bar: 120 µm (A) and 70 µm and magnified 7 µm (B). (C–E) Quantification of PCNA + , PCNA + /Fli + , and PCNA + /mpeg + cells in the SW hemisphere following phosphate-buffered saline (PBS) and VEGF165 microinjection. (F–H) Quantification of PCNA + , PCNA + /Fli + , and PCNA + /mpeg + cells in the SW hemisphere following DMSO and tivozanib microinjection. The data are presented as the mean ± standard error of the mean ( n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t -test. * P < 0.05, vs . the lesioned hemisphere of the respective control. DAPI: 4′,6-Diamidino-2-phenylindole; DMSO: dimethyl sulfoxide; EGFP: enhanced green fluorescent protein; mpeg: macrophage‐expressed gene; PBS: phosphate-buffered saline; PCNA: proliferating cell nuclear antigen; SW: stab-wounded; Vegf/VEGF: vascular endothelial growth factor.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Immunostaining, Injection, Saline, Microinjection, Control

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/NRR.NRR-D-23-01881

Figure Lengend Snippet: Overview of the pharmacological treatments performed in adult zebrafish

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Concentration Assay, Liposomes, Saline

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/NRR.NRR-D-23-01881

Figure Lengend Snippet: Analysis of regenerative angiogenesis and neurogenesis after VEGF165 injection. (A) PCNA immunohistostaining (proliferating cells in purple) in telencephalon sections of Tg( fli:EGFP × mpeg1.1:mCherry ) zebrafish (blood vessels in green) after microinjection with PBS (vehicle) and VEGF165 (50 µg/mL) at the SW injury site. Scale bars: 200 µm. (B–D) Quantification of different angiogenic parameters shows an increasing regenerative angiogenesis tendency in the SW hemisphere of VEGF165-injected zebrafish compared with the SW hemisphere of PBS-injected zebrafish at 1 dpl. (E) Quantification of the ventricular proliferative surface of fish injected with PBS and VEGF165 showing an increasing tendency for NSC proliferation. The data are presented as the mean ± standard error of the mean ( n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t -test. * P < 0.05, vs . the SW hemisphere of PBS-injected zebrafish. dpl: Day(s) post-lesion; EGFP: enhanced green fluorescent protein; GFP: green fluorescent protein; PBS: phosphate-buffered saline; PCNA: proliferating cell nuclear antigen; SW: stab-wounded; VEGF: vascular endothelial growth factor.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Injection, Microinjection, Saline

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/NRR.NRR-D-23-01881

Figure Lengend Snippet: Microglial recruitment is regulated by Vegf signaling. The SW hemisphere was compared with the respective contralateral hemisphere at 3 dpl. (A, C) Telencephalon sections of Tg( fli:EGFP × mpeg1.1:mCherry ) zebrafish (microglia in red). Scale bars: 200 µm. (B) Quantification of mpeg + cells showing a decrease in tivozanib-injected zebrafish compared with DMSO-injected zebrafish. (D) Quantification of mpeg + cells showing an increasing tendency in VEGF165-injected zebrafish compared with PBS-injected zebrafish. The data are presented as the mean ± standard error of the mean ( n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t -test. *** P < 0.001, vs . the contralateral hemisphere. DMSO: Dimethyl sulfoxide; dpl: day(s) post-lesion; mpeg: macrophage‐expressed gene; PBS: phosphate-buffered saline; SW: stab-wounded; Vegf/VEGF: vascular endothelial growth factor.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Injection, Saline

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/NRR.NRR-D-23-01881

Figure Lengend Snippet: Microglial and endothelial cell proliferation following brain lesion and modulation of Vegf signaling. (A) Representative images of PCNA immunostaining of Tg( fli:EGFP × mpeg1.1:mCherry ) zebrafish following microlesion/injection with DAPI counterstaining. (B) Higher magnification of the white panels in A illustrating endothelial cell proliferation (Fli + /PCNA + ) and microglial proliferation (mpeg + /PCNA + ). Scale bar: 120 µm (A) and 70 µm and magnified 7 µm (B). (C–E) Quantification of PCNA + , PCNA + /Fli + , and PCNA + /mpeg + cells in the SW hemisphere following phosphate-buffered saline (PBS) and VEGF165 microinjection. (F–H) Quantification of PCNA + , PCNA + /Fli + , and PCNA + /mpeg + cells in the SW hemisphere following DMSO and tivozanib microinjection. The data are presented as the mean ± standard error of the mean ( n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t -test. * P < 0.05, vs . the lesioned hemisphere of the respective control. DAPI: 4′,6-Diamidino-2-phenylindole; DMSO: dimethyl sulfoxide; EGFP: enhanced green fluorescent protein; mpeg: macrophage‐expressed gene; PBS: phosphate-buffered saline; PCNA: proliferating cell nuclear antigen; SW: stab-wounded; Vegf/VEGF: vascular endothelial growth factor.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Immunostaining, Injection, Saline, Microinjection, Control

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/nrr.nrr-d-23-01881

Figure Lengend Snippet: Figure 7 ｜Analysis of regenerative angiogenesis and neurogenesis after VEGF165 injection. (A) PCNA immunohistostaining (proliferating cells in purple) in telencephalon sections of Tg(fli:EGFP × mpeg1.1:mCherry) zebrafish (blood vessels in green) after microinjection with PBS (vehicle) and VEGF165 (50 µg/mL) at the SW injury site. Scale bars: 200 µm. (B–D) Quantification of different angiogenic parameters shows an increasing regenerative angiogenesis tendency in the SW hemisphere of VEGF165- injected zebrafish compared with the SW hemisphere of PBS-injected zebrafish at 1 dpl. (E) Quantification of the ventricular proliferative surface of fish injected with PBS and VEGF165 showing an increasing tendency for NSC proliferation. The data are presented as the mean ± standard error of the mean (n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t-test. *P < 0.05, vs. the SW hemisphere of PBS-injected zebrafish. dpl: Day(s) post-lesion; SW: stab-wounded; PBS: phosphate-buffered saline; PCNA: proliferating cell nuclear antigen; VEGF: vascular endothelial growth factor. EGFP: enhanced green fluorescent protein; GFP: green fluorescent protein.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Injection, Microinjection, Saline

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/nrr.nrr-d-23-01881

Figure Lengend Snippet: Figure 11 ｜Microglial and endothelial cell proliferation following brain lesion and modulation of Vegf signaling. (A) Representative images of PCNA immunostaining of Tg(fli:EGFP × mpeg1.1:mCherry) zebrafish following microlesion/injection with DAPI counterstaining. (B) Higher magnification of the white panels in A illustrating endothelial cell proliferation (Fli+/PCNA+) and microglial proliferation (mpeg+/PCNA+). Scale bar: 120 µm (A) and 70 µm and magnified 7 µm (B). (C–E) Quantification of PCNA+, PCNA+/Fli+, and PCNA+/ mpeg+ cells in the SW hemisphere following phosphate-buffered saline (PBS) and VEGF165 microinjection. (F–H) Quantification of PCNA+, PCNA+/Fli+, and PCNA+/mpeg+ cells in the SW hemisphere following DMSO and tivozanib microinjection. The data are presented as the mean ± standard error of the mean (n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t-test. *P < 0.05, vs. the lesioned hemisphere of the respective control. DMSO: Dimethyl sulfoxide; PBS: phosphate-buffered saline; SW: stab-wounded; Vegf/ VEGF: vascular endothelial growth factor; mpeg: macrophage‐expressed gene; DAPI: 4′,6-diamidino-2-phenylindole; EGFP: enhanced green fluorescent protein; PCNA: proliferating cell nuclear antigen.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Immunostaining, Injection, Saline, Microinjection, Control

Journal: Neural Regeneration Research

Article Title: Telencephalic stab wound injury induces regenerative angiogenesis and neurogenesis in zebrafish: unveiling the role of vascular endothelial growth factor signaling and microglia

doi: 10.4103/nrr.nrr-d-23-01881

Figure Lengend Snippet: Figure 10 ｜Microglial recruitment is regulated by Vegf signaling. The SW hemisphere was compared with the respective contralateral hemisphere at 3 dpl. (A, C) Telencephalon sections of Tg(fli:EGFP × mpeg1.1:mCherry) zebrafish (microglia in red). Scale bars: 200 µm. (B) Quantification of mpeg+ cells showing a decrease in tivozanib-injected zebrafish compared with DMSO-injected zebrafish. (D) Quantification of mpeg+ cells showing an increasing tendency in VEGF165-injected zebrafish compared with PBS-injected zebrafish. The data are presented as the mean ± standard error of the mean (n = 5–6 per condition from two independent experiments) and were analyzed with Student’s t-test. ***P < 0.001, vs. the contralateral hemisphere. DMSO: Dimethyl sulfoxide; PBS: phosphate-buffered saline; SW: stab- wounded; dpl: day(s) post-lesion; Vegf/ VEGF: vascular endothelial growth factor; mpeg: macrophage‐expressed gene.

Article Snippet: The positive effects of VEGF on angiogenesis and brain regeneration were investigated by injecting recombinant zebrafish VEGF165 at the lesion site: the fish received an intraparenchymal microinjection with 1× PBS or 50 μg/mL VEGF165 in 1× PBS (REF: 1247-ZV/CF, R&D Systems, Noyal Châtillon sur Seiche, France).

Techniques: Injection, Saline